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Sale Sold out. Error Quantity must be 1 or more. Add to cart. No more mess! This is a limited time discount. Discount does not apply to digital content. Discount good while supplies last or until withdrawn by Amazon. Shipping charges and taxes may apply to pre-discounted prices. Amazon reserves the right to modify or cancel the discount at any time. If any of the products or content related to this discount are returned, your refund will equal the amount you paid for the product or content, subject to applicable refund policies.

Enhance your purchase. About this item This fits your. Customer ratings by feature. Easy to clean. See all reviews. Similar item to consider. Buy it with. Total price:. To see our price, add these items to your cart. Some of these items ship sooner than the others. Show details Hide details. Choose items to buy together. Only 2 left in stock - order soon.

Customers who bought this item also bought. Page 1 of 1 Start over Page 1 of 1. Previous page. Woodlink 3 in 1 Platform Bird Feeder. Amazon's Choice. Next page. Here's how restrictions apply. From the manufacturer Audubon Feeders and Accessories. Proper Drainage wih Metal Mesh Tray The green powder-coated screen is easily removable for cleaning, and allows for proper drainage of your birdseed, and the ability to offer any type of birdseed, up to three pounds depending on seed variety.

Pole Mount Option with Wide Wood Strip It has an about 2 inch strip of thick wood across the bottom center suitable for attaching a pole mount. Compare with similar items. Product information Product Dimensions 13 x 13 x 3. Would you like to tell us about a lower price? Customer reviews. How customer reviews and ratings work Customer Reviews, including Product Star Ratings help customers to learn more about the product and decide whether it is the right product for them.

Learn more how customers reviews work on Amazon. Images in this review. Reviews with images. See all customer images. Top reviews Most recent Top reviews. Top reviews from the United States. There was a problem filtering reviews right now. Please try again later.

Verified Purchase. What it does have are 3 sturdy, extendable legs with true skid-proof pads on the bottom. The angle and position of the legs is well-designed - that is, zero tipping while my husky eats.

The removable part makes filling the bowl super easy. The design of this bowl really does cause a dog to eat more slowly. It's also what makes it a little trickier to clean, but it's not bad and definitely worth the trouble especially with dry kibble.

Extending the legs was a little stiff at first, but because they lock securely in the extended position, it's worth the one-time effort. I would definitely buy this bowl again. Thank you for an excellent product that works as it was designed to work. Our son has two labradoodles and one of them tends to eat very quickly and then throws up.

Not sure if it's because she's competing with her sister or just eats fast! This dish slows her down and has solved the problem. The dish is pretty wide and they actually eat together at the same time now. I keep one at our house for when we doodle sit and I throw it in the dishwasher when they go home.

It's a little difficult to wash by hand because of the grooves, but it does fine standing up on it's side in the dishwasher. We have the largest dish and the doodles are 25 pounds and 40 pounds. It takes about 10 minutes for my 8 month old German Shepard to eat 1 cup from this feeder. She will start to bite it and lift it a little so I monitor her. If she figures out to flip it to get the food fast, that will be the end of this.

Until then, this gives me a few minutes of peace while she licks out the food. We tried this when she was a little younger and it was too deep for a smaller tongue to get all the food out. So this is the 4th style bowl I've tried so far and this one works the best for my little pitt mix. She is a rescue dog that was very underweight so she gulps when she eats and then throws up.

I've tried the kind with swirls or bumps, but for some reason this design causes her eating to go from 10 seconds to about 3 minutes - no joke! I'd buy it asap and I'm ordering a second for a back up. I needed to slow my doberman down with her eating as she would eat her food in a flash and then hover over my other two dogs while they're trying to eat. This slow feed bowl is perfect. This slowed her down, and it seems she enjoys her food more and realizes that her stomach is full.

I am quite pleased with how well this bowl is working for us and would definitely recommend. Using for a German Shepherd puppy starting at 4 months old. He was a gulper and would hork down food in 10 seconds before got this.

Slowed him down nicely, still eats a little fast. I think he's learned some tricks on how to eat fast even with this. Two cups or more and he can hork down alot off the top pretty quickly. Still works and good quality. Our 6 month old puppy eats like she's been starved for weeks.

We got her a dish similar to this, but smaller initially. She quickly outgrew that one. I was worried this teal circular pattern would be too difficult to get food from, but she's got zero problems, lol! It's slowed her a little more than the first one, so that's a win. Top reviews from other countries. Translate all reviews to English. Je recommande ce petit investissement si vous avez un chat glouton, c'est tellement mieux pour lui Translate review to English.

My name is Hector. I am a puppy Rhodesian Ridgeback. My mum and dad bought this for me as I eat really quick and then burped and farted in their faces. Mum and dad like the bowl as it is easy to clean and it keeps me happy. The media could not be loaded. This item appears to be a cheap clone. There are no "Outward Hound" markings on the base or on the side, and no patent number, all present on a genuine Outward Hound fun feeder i already have.

The plastic is inferior, lightweight and the rubbery non slip section all round the base is also missing on both the ones i have received from Amazon so they slide all over the place.

I sent the first one back because of the missing non slip bit. I then looked at it more closely and noticed the other differences. The plastic looks like it would splinter if a dog picked it up and dropped it. The one I bought elsewhere is tough, dishwasher safe again markings on the bottom not present on these two and has a nice chunky solid feel to it. Definitely one to buy elsewhere and make sure you get the genuine article. This does exactly what it says on the tin.

I have 3 dogs, one of whom eats particularly quickly. It takes her less than 15 seconds to eat g of dry food. I've tried all sorts of measures including silicone mats with raised bobbly bits she's too clever for that - she just flips it over with her nose and tennis balls in her bowl she's too clever for that too - she just picks them out and drops them on the floor before inhaling her food. Transl Vis Sci Technol.

Generation of storable retinal organoids and retinal pigmented epithelium from adherent human iPS cells in xeno-free and feeder-free conditions. Differentiation of human embryonic stem cells into corneal epithelial progenitor cells under defined conditions. Skottman H. Derivation and characterization of three new human embryonic stem cell lines in Finland. Video image-based analysis of single human induced pluripotent stem cell derived cardiomyocyte beating dynamics using digital image correlation.

Biomed Eng Online. Comparative analysis of targeted differentiation of human induced pluripotent stem cells hiPSCs and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines. Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells.

Mol Vis. Structure and barrier properties of human embryonic stem cell-derived retinal pigment epithelial cells are affected by extracellular matrix protein coating. Tissue Eng Part A. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol. Chemically defined conditions for human iPSC derivation and culture.

Nat Methods. A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells. A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system. Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions.

Stem Cell Res. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Article Google Scholar. BCL-XL mediates the strong selective advantage of a 20q Co-ordinated ocular development from human iPS cells and recovery of corneal function. Cornea organoids from human induced pluripotent stem cells. Sci Rep. Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells.

Nat Protoc. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta Ophthalmol. Reconstruction of functional ocular surface by acellular porcine cornea matrix scaffold and limbal stem cells derived from human embryonic stem cells. Induction of epidermis and inhibition of neural fate by BMP Retinoic acid and bone morphogenetic protein signaling synergize to efficiently direct epithelial differentiation of human embryonic stem cells.

Engineering efficient retinal pigment epithelium differentiation from human pluripotent stem cells. Beta-catenin controls differentiation of the retinal pigment epithelium in the mouse optic cup by regulating Mitf and Otx2 expression. Fuhrmann S. Wnt signaling in eye organogenesis.

Human RPE melanosomes protect from photosensitized and iron-mediated oxidation but become pro-oxidant in the presence of iron upon photodegradation. Invest Ophthalmol Vis Sci. Stem cell derived retinal pigment epithelium: the role of pigmentation as maturation marker and gene expression profile comparison with human endogenous retinal pigment epithelium. Stem Cell Rev. Limbal stem-cell therapy and long-term corneal regeneration. Manufacturing, characterization and control of cell-based medicinal products: challenging paradigms toward commercial use.

Regen Med. Download references. We thank Professor Timo Otonkoski for providing the Hel The authors declare that all datasets supporting the conclusions of this article are available within the manuscript and its supplementary information files. You can also search for this author in PubMed Google Scholar. HH was responsible for the conception and design, developing and optimizing the differentiation protocols, acquiring and analyzing the data, and was the main contributor in manuscript writing.

TI was involved in conception and design, data analysis and interpretation, and manuscript writing. AM was involved in developing and optimizing the LESC differentiation, in designing experiments, collecting and interpreting the data, and in manuscript writing. HS was involved in conception and design, providing financial and administrative support, manuscript writing, and final approval of the manuscript.

All authors read and approved the final manuscript. Correspondence to Heidi Hongisto. No new cell lines were derived for this study. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. C Pluripotency shown as expression of markers of the three embryonic germ layers, namely SOX17 for endoderm, OTX2 for ectoderm, and SMA for mesoderm after spontaneous differentiation feeder-free passage level 4.

D Cells showing normal female karyotype after nine passages. TIF kb. C Pluripotency shown as expression of markers of the three embryonic germ layers after spontaneous differentiation feeder-free passage level 8.

Bright field BF images after eight passages. The arrow pointing out some differentiating cells. Counterstaining of nuclei with DAPI. In addition, the hiPSC1-RPE cells showed normal karyotypes after differentiation with and without neuroectodermal induction data not shown. Expression levels of undifferentiated hESCs UD, blue bars from the same culture system were used as a calibrator fold-change value set to 1 for each gene.

Relative fold-change values after embryoid body formation EB, red bars and after 4 or 6 days of induction green bars.

Reprints and Permissions. Hongisto, H. Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method.

Stem Cell Res Ther 8, Download citation. Received : 06 September

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